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Image Search Results
Journal: Biochemical Genetics
Article Title: High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis
doi: 10.1007/s10528-022-10262-z
Figure Lengend Snippet: Effect of circ_0001821 silencing on cell biological properties was counteracted by overexpression of ISOC1. A Overexpression efficiency of ISOC1 was examined by western blot assay. B – J SW620 and HCT116 cells were transfected with si-NC, si-circ_0001821, si-circ_0001821 + pcDNA or si-circ_0001821 + ISOC1. B and C The proliferative capacity of the cells was measured by colony formation assay and EDU assay. D and E The cell migration and invasion capabilities were assessed by transwell assay. F Flow cytometry was used to detect apoptosis of cells. G and H Glucose consumption and lactate production in CRC cell lines were tested. I and J Western blot assay was used to assess the protein expression of PCNA, MMP2, Bax and HK2. ** p < 0.01, *** p < 0.001
Article Snippet: Isochorismatase domain containing 1 (ISOC1) overexpression vector (ISOC1) and
Techniques: Over Expression, Western Blot, Transfection, Colony Assay, EdU Assay, Migration, Transwell Assay, Flow Cytometry, Expressing